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Supplementary Figure S5: Knockdown efficiency of siRNAs in METTL3.(A,B) The knockdown efficiency of METTL3 gene in U118 cells by three small interfering RNAs (siRNAs) were determined by qRT-PCR and Western blot (n = 3).(C,D) The knockdown efficiency of METTL3 gene in U78 cells by three small interfering RNAs (siRNAs) were …Introduction. During the last years, Neurofilament-light chain (NF-L) has been shown to be a valuable biomarker for several neurodegenerative diseases (Gaetani et al., 2019).Since NF-L is located in large caliber myelinated axons of neurons only, most clinical analyses were performed in the cerebrospinal fluid (CSF) of Multiple Sclerosis (MS) …Aug 10, 2010 · Also, a double-quoted string can contain apostrophes without backslashes, while a single-quoted string can contain unescaped quotation marks. Single quoted strings also use less memory. The fastest way to handle strings in PHP is with single quotes and using the . operator to concatenate strings and variables. 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